Core Laboratory Resources
The GCRC Core Lab offers the following capabilities:
Sample Processing- preparation and shipping of
research samples for delivery within the Health Center and to
external repositories and laboratories.
Immunoassays-
manually and
automated radio-immunoassay
and enzyme-linked immunoassay of hormone, bone marker, and
cytokine levels; serology; fluorescence activated cell sorting;
isolation of peripheral blood lymphocytes and cellular assay of
immune competence/reactivity
Nucleic Acid Assays- isolation of DNA and mRNA from
blood and tissue samples; RT-PCR analysis of specific mRNAs
using quantitative TaqMan PCR methods; human gene chip
microarray mRNA expression profiling; quantitative DNA copy
number evaluation for gene insertion/deletions;
PCR-based
genotyping; sequence identification of mutations in
disease-associated genes; coordination of peripheral blood
lymphocyte transformation by other GCRC Core Labs
Cell Culture- short-term culture of isolated blood
cells, bone marrow aspirates and fibroblasts. The Core Lab also
receives established lines of human cells and stores frozen
stocks of cell lines derived from research subjects for up to
three years. |
